Minimum Reactants Required for Synthesis of Skin and Peripheral Nerves; Extension to Tissues of Other Organs - Tissue and Organ Regeneration in Adults - page 183

Table 7.1 Reaction diagrams for synthesis of skin and of its tissues

No. Reactants Reaction conditions and structure of

product

References

Response of culture medium or defect in the absence of reactants (negative control for all reaction diagrams)
S01

Cell-free medium (in vitro) → no tissues
synthesized

Negative control for all reactions
in culture (in vitro); no tissues
synthesized

S02 Negative control for all reactions in

the adult dermis-free defect (in vivo);
epithelialized dermal scar synthesized

Billingham and Reynolds 1952;
Billingham and Medawar 1955; sev-
eral other authors; see Chap. 2

in vivo →

In vivo dermis-free defect

epithelialized dermal scar

A. Epidermis (E)
S1 KC + FB → E Lethally irradiated FB could be

replaced with medium from FB
culture; keratinizing epithelium
synthesized

Rheinwald and Green 1975a, b;
Green et al. 1979, 1981; O'Connor
et al. 1981; Regauer and Compton
1990

S2 KC → E KC cultured in defined medium, pH

5.6-5.8 and optimal KC density;
keratinizing epithelium synthesized

Eisinger et al. 1979; Peehl and Ham
1980; Tsao et al. 1982

S3 KC + DRT → E KC cultured on DRT; keratinizing

epithelium synthesized

Boyce and Hansbrough 1988

S4 KC + CBL → E KC cultured on collagenous bilayer;

keratinizing epithelium synthesized

Bosca et al. 1988; Tinois et al. 1991

S5 KC + FB + L-DRT → E Keratinocytes and fibroblasts cul-

tured with modified (laminated) DRT;
keratinizing epithelium synthesized

Cooper et al. 1991, 1993; Boyce et al.
1993

S6 KC + FB + COG → E KC cultured with FB in collagen gel;

keratinizing epithelium synthesized

Nolte et al. 1993, 1994; Hansbrough
et al. 1994b

B. Basement membrane (BM)
S7

KC + COG → E · BM

KC cultured on collagen gel; typi-
cally reported evidence for synthesis
of hemidesmosomes, lamina lucida,

Mann and Constable 1977; Hirone
and Taniguchi 1980; David et al.