Minimum Reactants Required for Synthesis of Skin and Peripheral Nerves; Extension to Tissues of Other Organs - Tissue and Organ Regeneration in Adults - page 185

Table 7.1 (continued)

No. Reactants Reaction conditions and structure of

product

References

S15 KC cultured on a collagen gel with

FB, then grafted; hemidesmosomes,
lamina lucida, lamina densa, anchor-
ing fibrils synthesized

Hansbrough et al. 1994b; Nolte et al.
1994

in vivo →

E · BM

KC + FB + COG

S16 KC + COFL → No BM KC cultured on collagen film; no BM

synthesized

Cook and Van Buskirk 1995

S17 KC + PL → No BM KC cultured on surface of plastic

dish; no BM synthesized

Cook and Van Buskirk 1995

S18 Uncultured KC seeded into DRT,

then grafted; continuous laminin,
collagen VII (anchoring fibrils),
α6β 4 integrin (hemidesmosomes)
synthesized

Compton et al. 1998

in vivo →

E · BM

KC + DRT

C. Dermis (D)
S19 Cell-free DRT grafted; synthesis of

thick, vascularized dermis with quasi-
randomly oriented collagen fibers; no
BM; no dermo-epidermal junction

Yannas 1981; Yannas et al. 1981,
1982a, b; Orgill 1983; Orgill et al.
1996; Orgill and Yannas 1998;
Compton et al. 1998

in vivo →

D

DRT

S20 KC + FB + COG → No D KC cultured on a collagen gel with

FB; no dermis synthesized

Bell et al. 1981a, 1983; Hull et al.
1983a

S21 KC cultured in vitro on collagen gel

with FB, then grafted; thick dermis
with basketweave pattern and vascu-
larization was synthesized

Bell et al. 1983; Hull et al. 1983b;
Hansbrough et al. 1994b; Nolte et al.
1994

in vivo →

E · BM · D

KC + FB + COG

S22 KC + CBL → No D KC cultured on collagenous bilayer;

no dermis synthesized

Bosca et al. 1988; Tinois et al. 1991

S23 KC + DRT → No D KC cultured with DRT; no dermis

synthesized

Boyce et al. 1988