Surface-Enhanced Raman Scattering-Based Bioimaging - Nanotechnology for Biomedical Imaging and Diagnostics - page 332

(a)

s

(b) (c)

Nontargeted

Targeted

5000 5000

Tumor

Tumor

3000

3000

1000 Liver

Liver 1000

400 800 1200

Raman shift (cm -1 )

1600 2000 400 800 1200

Raman shift (cm -1 )

1600 2000

(d)

785 nm laser beam

Tumor

Liver

Tail vein
injection Tumor

figure 11.8 Design, preparation, and properties of PEgylated gold nanoparticles for
in vivo tumor targeting and spectroscopic detection. (a) Preparation and schematic illus-
tration of the original gold colloid, a particle encoded with a Raman reporter and stabi-
lized with a protective layer of thiol-polyethylene glycol (thiol-PEg). In vivo cancer
targeting and SERS detection by antibody-conjugated gold nanoparticles that recognize
the tumor biomarker. SERS spectra obtained from the tumor and the liver locations by
using (b) targeted and (c) nontargeted nanoparticles. (d) Photographs showing the laser
beam (785 nm) focusing on the tumor site or on the anatomical location of the liver. The
Raman reporter molecule used was malachite green. (Reprinted with permission from
Ref. [18]. © Macmillan Publishers Ltd.)