Biomedical Engineering Reference
In-Depth Information
Estimated path
Real path
100
50
0
-50
-100
-
100
-50
-200
0
-150
-100
50
-50
x
(microns)
z
(microns)
100
0
Figure 10.11
Comparison between real and estimated path for real sequence.
simulated and the reconstructed paths for the linear and the coil path, respectively. The
results show very good agreement between the simulated values and the retrieved paths.
10.3.2 Experimental Results
We report results from tracking latex microspheres and cells in vitro. In the first
experiment, the spherical particle was displaced along the optical axis with known step to
create a situation similar to the linear path previously simulated. We made an experimental
calibration where a microscope liquid chamber with a fixed spherical latex particle was
translated axially along the
z
-direction with multiple and known steps. In
Figure 10.10
, the
images are recorded at different distances.
By applying the above equations (10.5) and (10.6), particle trajectory is calculated
estimating the centroid of the two microsphere images for each hologram recorded. The
result of this experiment is shown in
Figure 10.11
.
In the second experiment, we show how the method can be applied for 3D paths tracking of
multiple motile in vitro cells. A real experimental situation in which we had different cells
floating into a microfluidic chamber, without a priori information, before they fall to the
ground, i.e., before they attach to the bottom of the chamber. We recorded, with the
Search WWH ::
Custom Search