Nanopatterned Surfaces for Stem-Cell Engineering - Stem-Cell Nanoengineering - page 107

U-ES cells D-ES cells

OCT-4

SOX-2

(B)

U-ES cells

OCT-4

SOX-2

Figure 7.17 The expression of pluripotent markers. (A) Western blotting for Oct-4 and Sox-2
proteins in undifferentiated (U-ES cells) and differentiated (D-ES cells) mouse embryonic stem cells.
(B) Immunocytochemical staining for Oct-4 and Sox-2 proteins in undifferentiated mouse ES cells
(green channel). Cell nuclei were stained with 4',6-diamidino-2-phenylindole (blue). Figure reproduced
with permission from: ref. 56, © 2013 Elsevier. (See insert for color representation of the figure.)

Au

Si

Nano porous AAO mask

Peptide immobilization

RemovaI of AAO mask

Figure 7.18 Schematic illustrations of mask-assisted fabrication of peptide nanodots on an Au electrode
(left) and scanning electron microscopy images of the corresponding steps (right). Figure reproduced with
permission from: ref. 69, © 2010 Elsevier.