Biomedical Engineering Reference
In-Depth Information
Cys
- [Arg - Gly - Asp]
3
CRGD
Cys
- [Arg - Gly - Asp]
3
Cys
- [Arg - Gly - Asp]
3
Lys
CRGD-MAP
bAla
Cys
- [Arg - Gly - Asp]
3
Cys
- [Arg - Gly - Asp]
3
Lys
[Arg - Gly - Asp]
3
[Arg - Gly - Asp]
3
[Arg - Gly - Asp]
3
[Arg - Gly - Asp]
3
Lys
bAla -
Cys
RGD-MAP-C
Lys
Figure 7.15
Configuration of synthesized peptides including the organization of the amino acids
sequence. Figure reproduced with permission from: ref. 65, © 2008 Elsevier.
600
500
400
300
200
100
0
Bare
CRGD
CRGD-MAP
RGD-MAP-C
Figure 7.16
Cell proliferation on the various cysteine-modified oligopeptides. Figure reproduced
with permission from: ref. 65, © 2008 Elsevier.
in undifferentiated cells, but not in differentiated cells. Cho
et al.
[66] reported the appli-
cation of a nanoporous anodic alumina oxide (AAO) mask to fabricate an RGD peptide
[67, 68] nanopatterned surface by a self-assembly technique, using the AAO mask to
improve stem-cell adhesion on electrode surfaces, which directly affects the sensitivity of
electrochemical measurements. After confirmation of the peptide nanopatterned surface
and enhanced electrochemical signals from neural stem cells, the correlation between stem
cell viability and concentrations of malathion and
α
-cypermethrin was intensively studied
by both cyclic voltammetry (CV) and 3-[4, 5-dimethyl thiazol-2-y1]-2, 5-diphenyl tetrazo-
lium bromide (MTT) viability assay. Furthermore, Kafi
et al
. [69] have applied an AAO
mask to fabricate several peptide nanostructures, including Cys-(RGD)
4
, RGD-MAP-Cys
peptide, and poly-l-lysine (PLL) nanodots on a gold surface as a neural cell chip
(Figure 7.18). The RGD-MAP-Cys peptide nanopatterned surface provided the strongest
voltammetric signals after 48 h of incubation (Figure 7.19), which could be due to an
