Biomedical Engineering Reference
In-Depth Information
(A)
(B)
Neuron
2
(i)
e
-
e
-
1
Applying
reduction potential
Applying
oxidation potential
0
5.0
(ii)
Bare Au
-1
2.5
0.0
-2.5
-5.0
PC12 cell
40 mV
-2
Au
Au/C(RGD)
4
Au/PLL
-3
Au/RGD-MAP-C
350 mV
-7.5
-4
0.8
0.6
0.4
E
/V vs. Ag/AgCl
0.2
0.0
-0.2
0.8
0.6
0.4
0.2
0.0
-0.2
E
/V vs. Ag/AgCl
(C)
(D)
6
5
4
2
4
0
3
-2
-4
2
-6
Au/C(RGD)
4
/Cell
Au/RGD-MAP-C/Cell
1
-8
Au/PLL/Cell
-10
0
0.8
0.6
0.4
0.2
0.0
-0.2
E
/V vs. Ag/AgCl
Figure 7.19
The arginine-glycine-aspartate multiple-arm peptides cysteine (RGD-MAP-Cys)
nanopatterned surface and voltammetric signals. (A) Schematic representation of (i) redox
phenomena at the cell-substrate interface and (ii) the redox peaks obtained from a PC12 cell
immobilized on a peptide-fabricated Au electrode. (B) Cyclic voltammetry for comparing the
electrochemical signal using Cys(RGD)
4
, RGD-MAP-Cys, and poly-l-lysine (PLL) fabricated Au
electrodes without a PC12 cell. (C) Cyclic voltammetry for comparing the electrochemical signal
using Cys(RGD)
4
, RGD-MAP-Cys, and PLL fabricated Au electrodes with a PC12 cell. (D)
Comparison between reduction peak current (
I
pc
) from PC12 cells on the Cys(RGD)
4
, RGD-MAP-
Cys and PLL patterned Au surfaces. Cyclic voltammetry was measured using phosphate buffer
solution (0.01
m
, pH 7.4) as the electrolyte at a scan rate of 100 mV s
−1
and the whole experiment
was conducted at a temperature of 27 ± 1 °C using Pt and Ag/AgCl as counter and reference
electrodes respectively. Each signal was obtained from the average value of three independent
measurements. Figure reproduced with permission from: ref. 69, © 2010 Elsevier.
(See insert for
color representation of the figure.)
enhanced affinity between the cells and the Au surface. In addition, Kang's group [70] fab-
ricated a cell chip using immobilized RGD-MAP-Cys peptides on the surface of an Au
electrode to measure the toxicity of GO nanopellets in human NSCs (HB1.F3 cells) [68,
71]. The cells were treated with different concentrations of GO and its toxicity was con-
firmed through differential pulse voltammetry (DPV). The DPV results demonstrated
