Chemistry Reference
In-Depth Information
Figure 2.5 Sub-steps within a displacement of myosin-II [17, 25].
(a) and (b) Time courses of displacements (upper traces) and
rising phases of displacements in expanded time scales
(lower traces) at low loads and high loads, respectively.
(c) Number of sub-steps per displacement at low and high loads.
(d) Ratio of forward and backward sub-steps.
myosin in solution [27] and in optical trapping nanometry [28]. The results indicate
that each displacement corresponds to one cycle of ATP turnover, consistent with the
observation of Cy3 attached to myosin on the tip of a scanning probe.
2.3.3
Sub-steps within a Displacement
Figure 2.5a and b (lower trace) show the rising phases of the displacements at low
(0.01
-
0.1 pN/nm) and high (0.1
-
0.6 pN/nm) needle stiffness on an expanded time
