Biomedical Engineering Reference
In-Depth Information
materials were modii ed consecutively with 3-aminopropyltrimethoxysi-
lane and maleic anhydride to introduce polymerizable double bonds and
terminal carboxyl groups, hence capable of physically adsorbing the print
protein. With lysozyme as a template, thin protein-imprinted shells were
fabricated according to this newly developed approach for surface protein
imprinting over nanoparticles. h e rebinding experiments coni rmed
that the introduction of the carboxyl groups could remarkably improve
the imprinting ef ect in relation to a signii cantly increased imprinting
factor and specii c rebinding capacity. Moreover, in contrast to the harsh
template removal conditions required for the covalent template coupling
approach, the template removal during the imprinted particle synthesis
as well as desorption at er rebinding could be mildly achieved via wash-
ing with salt solution.
Chemical nanosensors with a submicrometer core-shell composite
design, based on a polymer core, a MIP shell for specii c analyte recogni-
tion, and an interlayer of gold nanoparticles for signal amplii cation, were
also reported by Bompart
et al.
[37]. Surface-enhanced Raman scatter-
ing (SERS) measurements on single nanosensors yield detection limits of
10
−7
M for the
β
-blocker propranolol, several orders of magnitude lower
than on plain MIP spheres.
Gültekin
et al.
, have proposed a novel thiol ligand-capping method
with polymerizable methacryloylamido-cysteine (MAC) attached to
gold-silver nanoclusters, reminiscent of a self-assembled monolayer
and have reconstructed surface shell by synthetic host polymers based
on molecular imprinting method for recognition [38]. In this method,
methacryloylamidoantipyrine-terbium (MAAP)
2
-Tb(III) has been used
as a new metal-chelating monomer via metal coordination-chelation
interactions and dipicolinic acid (DPA) which is main participant of
Bacillus cereus spores used as a model. Nanoshell sensors with tem-
plates give a cavity that is selective for DPA. h e DPA can simultane-
ously chelate to Tb(III) metal ion and it into the shape-selective cavity.
h us, the interaction between Tb(III) ion and free coordination spheres
has an ef ect on the bindingability of the gold-silver nanoclusters nano-
sensor. h e binding afinity of the DPA imprinted nanoclusters has been
investigated by using the Langmuir and Scatchard methods, and the
respective afinity constants determined were found to be 1.43×10
-4
and
9.1×10
-6
mol L
-1
.
Recently Chen
et al.
have reported a sensitive and selective electro-
chemical sensor for metronidazole (MNZ) [39]. h e core-shell metro-
nidazole-m-MIP was synthesized and then attached to the surface of
magnetic glassy carbon electrode (MGCE) with the help of magnetic
