Biomedical Engineering Reference
In-Depth Information
gold nanoparticles nanosensor. h e interactions between DPA and MIP
particles were studied observing l uorescence measurements. DPA addi-
tion caused signii cant decreases in l uorescence intensity because they
induced photoluminescence emission from Au nanoparticles through the
specii c binding to the recognition sites of the cross-linked nanoshell poly-
mer matrix. h e binding ai nity of the DPA imprinted nanoparticles has
been explored by using the Langmuir and Scatchard methods and the anal-
ysis of the quenching results has been performed in terms of the Stern-
Volmer equation.
Combination of surface molecular imprinitng with sol-gel pro-
cess was reported by Chen
et al.
, for the preparation of core-shell mag-
netic nanoparticle-based metronidazole-imprinted polymer. Chen
et al.
described imprinting onto the surface of magnetic nanoparticle using
3-aminoprophyltriethoxysilane as the functional monomer, and tetra-
ethyl orthosilicate as the cross-linker [34]. h e adsorbent can be applied to
the solid phase extraction of metronidazole and exhibits high adsorption
capacity, good selectivity, favourable reusability and the feature of magnetic
separation. h e binding performances of the adsorbent were evaluated by
equilibrium rebinding experiments and Scatchard analysis. h e material
was successfully applied to solid phase extraction of metronidazole, fol-
lowed by spectrophotometric determination in food samples. In another
work, the use of core-shell imprinted nanoparticles for the selective rec-
ognition of thifensulfuronmethyl (TFM) using an electrochemilumines-
cence (ECL) method was reported by Li
et al.
h e core-shell imprinted
nanoparticles were i rst prepared by a surface monomer-directing strategy
for imprinting TFM at the surface of 3- methacryloxypropyl trimethoxysi-
lane modii ed silica particles [35]. h en, the ECL sensor was prepared by
depositing the core-shell imprinted nanoparticles/chitosan composite i lm
on the bare glassy carbon electrode surface and further removing silica
cores from the composite i lm. h e electrochemical and ECL behaviors
of luminol at the sensor were investigated in the absence and presence of
TFM. It was also found that the ECL intensity could be strikingly enhanced
by the adsorbed TFM molecules in the composite i lm, which was about
2.7-fold as compared with the blank ECL intensity.
Surface molecular imprinting, in particular over nanosized support
materials, is very suitable for a template of bulky structure like protein
[36]. Inspired by the surface template immobilization method reported
previously, Fu
et al.
demonstrate an alternative strategy for enhanc-
ing specii c recognition of core-shell protein-imprinted nanoparticles
through prefunctionalizing the cores with noncovalent template sorption
groups. For proof of this concept, silica nanoparticles chosen as the core
