Biomedical Engineering Reference
In-Depth Information
force in order to prepare m-MIP/MGCE sensor [39]. h e two very popu-
lar techniques CV and EIS have been applied to investigate the perfor-
mance of the obtained imprinted sensor. Various factors, known to af ect
the response behavior of the MMIP/MGCE electrode, were studied and
optimized. h e imprinted sensor exhibits high recognition ability and
ai nity for MNZ in comparison with the non-imprinted one. In addition,
the MMIP/MGCE also shows good stability and acceptable reproduc-
ibility for the determination of MNZ. Under the optimal experimental
conditions, the current response of the electrochemical sensor was linear
to MNZ concentrations in the range from 5.0×10
-8
to 1.0×10
-6
M, with
the detection limit of 1.6×10
-8
M. h e method was successfully applied to
the analysis of MNZ in milk samples and honey samples with acceptable
recoveries of 93.5% to 102.2%.
Similarly, Ma
et al.
, prepared a core-shell molecularly imprinted
polymers of 17β-estradiol on the surface of silica nanoparticles (SiO
2
@
E2-MIPs) [40]. h e sorption capacity of the SiO
2
@E2-MIPs were nearly
5 times that of the NIPs, and it only took 25 min to achieve the sorption
equilibrium. It indicated that the SiO
2
@E2-MIPs exhibited a high selectiv-
ity, large adsorption capacity and fast kinetics. When the SiO
2
@E2-MIPs
were used as dispersive solid-phase extraction absorbents to selectively
enrich and determine estrogens in duck feed, the average recoveries of E2
and estriol (E3) were higher than 96.74% and 72.07%, respectively, and the
relative standard deviations (RSD) of E2 and E3 were less than 1.61% and
3.28%, respectively. h e study provides an ef ective method for the separa-
tion and enrichment of estrogens in the complex matrix samples by the
SiO
2
@E2-MIPs.
In general, SiO
2
based core shell nanoparticle are more preferable and
reported in a lot, but some core-shell gold nanoparticle are also reported as
a substrate for imprinting. In this year 2013, Xue
et al.
explored a surface-
imprinted core-shell AuNPs for the highly selective detection of BPA by
SERS [41]. A triethoxysilane-template complex (BPA-Si) was synthesized
and then utilized to fabricate a MIP layer on the AuNPs via a sol-gel pro-
cess (Figure 11.15).
The imprinted BPA molecules were removed by a simple thermal
treatment to generate the imprint-removed material, MIP-ir-AuNPs,
with the desired recognition sites that could selectively rebind the BPA
molecules. The morphological and polymeric characteristics of MIP-
ir-AuNPs were investigated by transmission electron microscopy and
Fourier-transform infrared spectroscopy. The results demonstrated
that the MIP-ir-AuNPs were fabricated with a 2nm MIP shell layer
