Biomedical Engineering Reference
In-Depth Information
the magnetization was 14.8 emu/g. h e activities of both bound template
and rebound enzyme was established by measuring glucose production via
starch hydrolysis, at dif erent temperatures, for MIPs with dif erent com-
positions (wt% polymer and mol% ethylene). h e highest hydrolysis activ-
ity of m-MIPs (obtained with 32 mol% ethylene) was found to be 1545.2
U/g. Compared to the conventional catalysis process, m-MIPs have the
advantages of high surface area, suspension, easy removal from reaction,
and rapid reload of enzyme.
In the area of macromolecules separation and enrichment imprinting is
in under-develped stage, due to several dii culties like structural changes,
pH of the medium, solubility of macromolecules etc. Gao
et al.
, have been
reported imprinting of four proteins with dif erent isoelectric point {bovine
serum albumin (BSA, pI=4.9), bovine hemoglobin (BHb, pI=6.9), bovine
pancreas ribonuclease A (RNase A, pI=9.4) and lysozyme (Lyz, pI=11.2)}
onto the surface of MNPs with a uniform core-shell structure for the rec-
ognition and enrichment of protein was developed [23]. h e magnetic pro-
tein-MIPs were synthesized by combining surface imprinting and sol-gel
techniques. In comparison with the use of Lyz, BSA and RNase A as tem-
plate proteins, BHb-imprinted Fe
3
O
4
showed the best imprinting ef ect and
the highest adsorption capacity among the four proteins. h e as-prepared
Fe
3
O
4
@BHb-MIPs NPs with a mean diameter of 230 nm were coated with
an MIP shell that was 10 nm thick, which enabled the Fe
3
O
4
@BHb-MIPs
to easily reach adsorption equilibrium. A high magnetic saturation value
of 25.47 emu g
-1
for Fe
3
O
4
@BHb-MIPs NPs was obtained, which endowed
the adsorbent with the convenience of magnetic separation under an exter-
nal magnetic i eld. h e resultant Fe
3
O
4
@BHb-MIPs NPs could not only
selectively extract a target protein from mixed proteins but also specii cally
capture the protein BHb from a real sample of bovine blood. In addition, dif-
ferent batches of magnetic MIPs showed good reproducibility and reusabil-
ity for at least six repeated cycles. Emulsion polyemrization techniques were
also used for the fabrication of m-MIP. Another BHb-imprinted polysty-
rene (PS) nanoparticles with magnetic susceptibility have been synthesized
through multistage core-shell polymerization system using 3-aminophenyl-
boronic acid (APBA) as functional and cross-linking monomers by Lin
et al
[24]. Super paramagnetic molecularly imprinted polystyrene nanospheres
with poly (APBA) thin i lms have been synthesized and used for the i rst
time for protein molecular imprinting in an aqueous solution. h emagnetic
susceptibility is imparted through the successful encapsulation of Fe
3
O
4
nanoparticles. h e imprinted super paramagnetic nanoparticles could eas-
ily reach the adsorption equilibrium and achieve magnetic separation in an
external magnetic i eld, thus avoiding some problems of the bulk polymer.
