Biomedical Engineering Reference
In-Depth Information
Figure 11.7
Schematic representation of nanoshell based on p-nitrophenyl palmitate-
template constructed onto the surface of iron oxide nanoparticles [Co-opted from
reference 19].
Jia
et al.
synthesized a polydopamine-based molecular imprinted i lm
coating on silica-Fe
3
O
4
nanoparticles for recognition and separation of
bovine hemoglobin. Herein, m-MIP having diameter (860nm) show a rela-
tively high adsorption capacity (4.65 ± 0.38 mg g
-1
) and excellent selectivity
towards bovine hemoglobin with a separation factor of 2.19. m-MIP with
high saturation magnetization (10.33 emu g
-1
) makes it easy to separate
the target protein from solution by an external magnetic i eld. At er three
continuous adsorption and elution processes, the adsorption capacity of
m-MIP remained at 4.30 mg g
-1
. Similarly, a novel super paramagnetic
surface molecularly imprinted Fe
3
O
4
@MIP nanoparticles for water-solu-
ble pel oxacin mesylate were prepared via surface initiated atom transfer
radical polymerization [21]. h e Fe
3
O
4
@MIP exhibited high saturation
magnetization of 41.4 emu/g leading to the fast separation. h e adsorption
behaviours indicated that the Fe
3
O
4
@MIP nanoparticles possessed specii c
recognition and high ai nity towards template in aqueous media.
Phase inversion method was also applied in some case to prepare m-MIP
for dif erent macromolecules like enzymes and protein. Lee
et al.
, have
reported a amylse imprinted m-MIP using phase inversion of poly (ethyl-
ene-co-vinyl alcohol) solutions with 27−44 mol% ethylene in the presence
of amylase [22]. h e mean size of m-MIPs was found to be 100 nm and
