Biomedical Engineering Reference
In-Depth Information
Gu
et al.
, have reported chlorogenic acid imprinted MNPs via water-
in-oil-in-water multiple emulsions suspension polymerization [25]. h is
kind of m-MIPs had the core-shell structure with the size of about 50 nm.
Magnetic susceptibility was given by the successful encapsulation of Fe
3
O
4
nanoparticles with a high encapsulation ei ciency of 19.3 wt%.
Super paramagnetic nanoparticles are of great current interest for bio-
medical applications in both diagnostics and treatment. Lee
et al.
, have
reported Albumin, creatinine, lysozyme and urea-imprinted polymer
nanoparticles from poly (ethylene-
co
-ethylene alcohol) via phase inver-
sion, with both target molecules and hydrophobic magnetic nanoparticles
mixed within the polymer solution [26]. h e composite m-MIP were used
for separation and sensing of template molecules (e.g., human serum albu-
min) in real samples (urine).
A Redox-active m-MIP nanospheres were i rst synthesized and func-
tionalized with streptomycin templates for highly ei cient electrochemical
determination of streptomycin residues (STR) in food by coupling with
bioelectrocatalytic reaction of enzymes for signal amplii cation by Liu
et al
[27]. h e m-MIP nanospheres were synthesized by using Au(III)-
promoted molecularly imprinted polymerization with STR templates on
magnetic beads (Figure 11.8).
Based on extraction of template molecules from them MIP surface, the
imprints toward STR templates were formed. h e assay was implemented
magnetic bead
nanogold particle
poly (OPD)
STR template
Imprinting cavity
Electrocatalytic
reaction
Potential vs.Ag/AgCl (V)
reusage
STR-GOX
STR-GOX
without analyte
ITO electrode
STR analyte
magnet
strong signal
mMIP-based sensor
weak signal
Figure 11.8
Schematic illustration of nanogold-promoted magnetic molecularly
imprinting polymer nanospheres for competitive-type electrochemical detection of
streptomycin (STR) residues by coupling with bioelectrocatalytic reaction of glucose
oxidase (GOX) for signal amplii cation [Co-opted from reference 27 with permission].
