Chemistry Reference
In-Depth Information
N
Cl
N
O
N
O
O
S
λ
abs
= 372 nm;
λ
em
=ca. 429 nm after
derviatisation with
aliphatic amines,
chloroform
NO
2
λ
abs
= 336 nm;
λ
em
= ca. 535 nm after
derviatisation with
aliphatic amines,
methanol
Cl
fIgure 11.20
Structures of NBD chloride (left) and dansyl chloride (right).
+
N
-
N
B
F
F
The BODIPY luminophore
BODIPY-based dyes
λ
abs
(nm)
λ
em
(nm)
Remarks
BODIPY FL
503
512
/
BODIPY R6G
528
547
/
BODIPY TMR
543
569
/
BODIPY TR
592
618
/
BODIPY 630/650
625
640
/
BODIPY 650/665
646
660
/
Oxidative responsive probe with
λ
em
shifts from ∼ 590 nm to ∼ 510
nm upon oxidation of the
unsaturated butadienyl portion
BODIPY 581/591
∼ 510 to ∼ 590
fIgure 11.21
Structure of BODIPY.
Photo-induced electron transfer (PET) quenching
O
HN
N
H
2
N
+
O
FHIT
N
N
N
-
O
O
O
N
O
+
B
N
N
-
O
O
F
F
NH
S
P
O P
O P
O
B
O
F
F
NH
S
P
O
P
OH
O
O
O
-
-
-
O
O
-
-
HO
OH
BODIPYFL thiodiphosphate
(strongly uorescence)
O
HN
N
H
2
N
N
O
N
HO
P
O
O
GMP
O
-
HO
OH
fIgure 11.22
Specific responsive properties of BODIPY FL GTP-γ-
S.
