Biomedical Engineering Reference
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content of the target protein in TB plus medium did not depend on the time of addition of
nalidixic acid and, on the average, was 16.5 ± 0.4 % of total cellular proteins. At the third
stage the cultivation process of
E.coli
JM 103/pTBI was transfered from shaken flasks to a
laboratory fermenter. The parameters of fermentation were chosen in accordance with the
results of previous experiments that allowed us to enhance the content of the target protein.
TB plus media was chosen as a cultivation medium, TB medium served as a control.
Nalidixic acid was added at an optical density of 0.8-1.0. Air was passed as earlier described.
Figure 5. The growth curves of
E.coli
JM 103/pTBI and accumulation of TBI protein in optimized
media after induction at an optical density of 1.5. A - Growth curves. B - The dynamics of
accumulation of TBI protein.
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