Biomedical Engineering Reference
In-Depth Information
Figure 6. The growth curves of
E.coli
JM 103/pTBI in a laboratory fermenter.
The dynamics of cell concentration and the content of TBI protein were analyzed in the
process of fermentation. Three fermentation processes were carried out in TB plus medium
and one - in TB medium.
The first fermentation was carried out in TB medium. In this case nalidixic acid was
added at an optical density of 0.9. Whereas the yield of cells was 19.6 g the content of the
protein in biomass was lower than in flasks and amounted to 13.3 %.
The second fermentation was carried out in TB plus medium. Nalidixic acid was added at
an optical density of 1.0.
The yield of cells was 21.8 g and the content of the protein in biomass - 16.4 %, which
corresponded to the data obtained during cultivation in flasks. To assess the influence of the
time of addition of nalidixic acid on the concentration of cells and the content of protein in
biomass the subsequent fermentation processes were carried out with adding nalidixic acid at
an optical density of 0.8.
It was shown that the content of protein in biomass amounted to 18.2 % and 17.4 % at
practically the same concentration of cells. The yield of cells constituted 15.4 g and 16.0 g,
respectively, and was lower in comparison with the second fermentation.
Thus, the content of TBI protein in biomass remained high and did not depend on the
time of addition of nalidixic acid in TB plus medium, which corresponded to the data
obtained in cultivation flasks.
Search WWH ::
Custom Search