Biomedical Engineering Reference
In-Depth Information
Figure 4. The growth curves of
E.coli
JM 103/pTBI and accumulation of TBI protein in optimized
media after induction at an optical density of 1.2. A - Growth curves. B - The dynamics of
accumulation of TBI protein.
At the second stage the dynamics of accumulation of the target protein was studied
depending of the time of addition of nalidixic acid. Cultivation was carried out in Erlenmeyer
flasks using optimized TB and TB plus media. The culture was grown to an optical density of
0.8, 1.2 and 1.5. Then nalidixic acid was added to a final concentration of 90 µg/ml. Optical
density was measured every hour. Following induction the content of recombinant TBI
protein was determined. The data obtained are presented in Figures 3, 4, 5.
It is seen that at the end of cultivation the concentration of cells did not depend on the
composition of the medium and the time of addition of the inducer. The protein content in TB
plus medium was higher than in TB medium. The maximal content of TBI protein constituted
15.6 % in TB medium in the case of addition of the inducer at an optical density of 0.8. The
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