Biomedical Engineering Reference
In-Depth Information
Figure 3. The growth curves of
E.coli
JM 103/pTBI and accumulation of TBI protein in optimized
media after induction at an optical density of 0.8. A - Growth curves. B - The dynamics of
accumulation of TBI protein.
The process of cultivation was carried out as described in Materials and Methods. The
culture was grown up to an optical density of 0.7-0.8, then nalidixic acid was added to a final
concentration of 90 µg/ml. Optical density was measured every hour. Following induction the
content of recombinant TBI protein was determined.
The data obtained are presented in Figures 1, 2. It was demonstrated that despite the
absence of differences in cell concentration the content of recombinant TBI protein in
optimized media was significantly higher as compared to the control. The amount of TBI
protein was 15.8 ± 0.5 % in TB medium and 16.2 ± 0.4 % in TB plus medium versus 8.1 ±
0.4 % in YT medium.
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