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Table 12.2 Hematological indices and activities of antioxidative enzymes in dogs with CanVL
after Sb
v
therapy (
T
60
) and in healthy dogs (CTRL)
T
60
Mean
SD (C.I.
95%
)
CTRL Mean
SD (C.I.
95%
)
P
0.05
<
RBC (x106/
L)
5.49
0.8 (4.98-6.0)
6.94
0.61 (6.68-7.2)
0.0001
m
<
HGB (g/dL)
12.46
2.0 (11.19-13.73)
16.86
1.67 (16.16-17.57)
0.0001
<
HCT (%)
35.43
5.41 (32.0-38.87)
47.65
4.55 (45.72-49.57)
0.0001
<
MCV (fL)
64.58
3.4 (62.42-66.75)
68.58
2.78 (67.41-69.76)
0.001
<
MCH (pg)
22.72
1.8 (21.58-23.86)
24.3
1.28 (23.76-24.84)
0.01
<
MCHC (g/dL)
35.15
1.57 (34.15-36.15)
35.43
2.22 (34.49-36.37)
n.s.
SOD (U/gHGB)
115.2
21.6 (101.5-129.0)
82.6
9.0 (78.8-86.4)
<
0.0001
GSHPx (U/L)
872.1
271.9 (699.3-1044.8)
1025.3
284.6 (905.2-1145.5)
n.s.
(Britti et al.
2008
). Particularly, simple linear regression analysis indicated a strong
negative linear associations between SOD activity and HGB concentration
(
R
2
<
0.0001), SOD activity and RBCs (
R
2
¼
0.96;
P
¼
0.76;
P
<
0.0001), and
SOD activity and HCT (
R
2
0.0001) at
T
60
. No significant associations
were detected at
T
60
between SOD and GSHPx activities and between GSHPx
activities and hematological indices. Additionally, a minimal negative association
(
R
2
0.90;
P
¼
<
0.05) was detected between SOD activities at
T
0
and HGB
concentrations at
T
60
, and a minimal positive association (
R
2
0.38;
P
¼
<
0.05)
was detected between GSHPx activities at
T
0
and HGB concentrations at
T
60
.
Finally, a multiple linear regression analysis was used to develop a moderately
accurate algorithm (
R
2
0.39;
P
¼
<
0.05) to predict HGB concentrations at
T
60
by
measuring SOD and GSHPx activities at
T
0
. The relationship between these
variables (in the absence of colinearity between SOD and GSHPx at
T
0
) is described
by the following formula: HGB
T60
(g/dL)
0.50;
P
¼
<
13.901 + 0.004
GSHPx
T0
(U/L)
¼
0.045
SOD
T0
(U/gHGB). In the developed algorithm, parameters of regression
indicate a positive association between GSHPx
T0
activities and HGB
T60
concentrations and a negative association between SOD
T0
activities and HGB
T60
concentrations.
12.4 Discussion
Results of our study revealed that the presence or absence of a previous treatment
with Sb
v
had no statistically identifiable effect on antioxidative enzyme activities
and hematological indices. No significant differences in SOD and GSHPx activities
and hematological indices were detected in dogs with CanVL before and after
therapy with Sb
v
(
T
0
and
T
60
). Erythrocyte SOD activities were significantly higher
and HGB, RBCs, HCT, MCV, and MCH levels were significantly lower in dogs
with CanVL at 60 days after therapy as compared to clinically healthy dogs
(Figs.
12.1
and
12.2
). On the basis of these findings, we assume that these
parameters are significantly altered in dogs with CanVL as compared with those
of control dogs, regardless of the Sb
v
treatment. This result suggests that in dogs
