Biology Reference
In-Depth Information
jugular venopuncture and divided into an EDTA tube for CBC analysis
(Advia120
®
) and a LiHe tube for the measurement of antioxidative enzyme
activities. Particularly, SOD activity was measured after isolation and lysis of
RBCs, while GSHPx activity was determined in whole blood. Tests were performed
using a clinical chemistry analyzer (Olympus-AU400
®
), according to the respective
manufacturer's specification (Ransod
®
and Ransel
®
, RandoxLab).
Shapiro-Wilk tests indicated that the data followed a normal distribution. The
Student's
t
-tests were used to evaluate the differences in the mean values of
antioxidative SOD and GSHPx activities and hematological indices, including
HGB, RBCs, HCT, MCV, MCH, and MCHC between
T
0
and
T
60
groups (paired
data) and between
T
0
or
T
60
and CTRL groups (unpaired data). Linear regression
analysis was used to evaluate the potential associations between these variables
(pre- and posttreatment). All calculations were performed using a commercial
statistical package (GraphPad Prism
®
).
12.3 Results
Means
SDs, 95% confidence intervals (CIs
95%
) of the means, and
P
values for
testing the differences between groups are reported in Tables
12.1
and
12.2
. The
comparison between
T
0
and CTRL groups was reported in our previous study (Britti
et al.
2008
). In the current study, the comparison between
T
0
and
T
60
groups
revealed no significant differences in the mean values for SOD and GSHPx
activities and the hematological indices, including HGB, RBCs, HCT, MCV,
MCH, and MCHC levels. For example, no significant differences were detected
between pre- and posttreatment with Sb
v
for each of the considered variables in this
study (Table
12.1
). The comparison between
T
60
and CTRL groups revealed a mean
level of SOD activity significantly higher (
P
0.05) at
T
60
and mean levels of
HGB, RBCs, HCT, MCV, and MCH significantly lower (
P
<
0.05) at
T
60
.No
significant differences were detected for GSHPx and MCHC levels (Table
12.2
).
Simple linear regression analyses demonstrated significant negative associations
among the variables at
T
60
, similar to those detected at
T
0
in our previous study
<
Table 12.1 Hematological indices and activities of antioxidative enzymes in dogs with CanVL
before (
T
0
) and after (
T
60
)Sb
v
therapy
T
0
Mean
SD (C.I.
95%
)
T
60
Mean
SD (C.I.
95%
)
P
0.05
<
RBC (x106/
L)
5.64
0.83 (5.11-6.17)
5.49
0.8 (4.98-6.0)
n.s.
m
HGB (g/dL)
12.42
2.04 (11.12-13.71)
12.46
2.0 (11.19-13.73)
n.s.
HCT (%)
35.67
6.01 (31.86-39.49)
35.43
5.41 (32.0-38.87)
n.s.
MCV (fL)
60.99
9.2 (55.15-66.84)
64.58
3.4 (62.42-66.75)
n.s.
MCH (pg)
21.69
2.24 (20.27-23.11)
22.72
1.8 (21.58-23.86)
n.s.
MCHC (g/dL)
33.78
4.44 (30.95-36.6)
35.15
1.57 (34.15-36.15)
n.s.
SOD (U/gHGB)
114.2
18.0 (102.7-125.6)
115.2
21.6 (101.5-129.0)
n.s.
GSHPx (U/L)
988.7
222.3 (847.5-1130.0)
872.1
271.9 (699.3-1044.8)
n.s.
