Superoxide Dismutase and Glutathione Peroxidase in Dogs with Leishmaniasis Following Antimoniate Therapy - Veterinary Science - page 75

Fig. 12.1 Dots represent
individual HGB (g/dL)
values. Horizontal lines
represent mean SD.
A ¼ T 0 ;B ¼ T 60 ;
C ¼ CTRL. * P < 0.05 as
compared to CTRL

20.0

* *

17.5

15.0

12.5

10.0

7.5

A B C

Fig. 12.2 Dots represent
individual erythrocyte SOD
(U/gHGB) values. Horizontal
lines represent mean SD.
A ¼ T 0 ;B ¼ T 60 ;
C ¼ CTRL. * P < 0.05 as
compared to CTRL

*

*

180

160

140

120

100

80

60

A B C

with CanVL, Sb v therapy does not improve the redox state or the underlying
anemia. This may be explained, at least in part, by other antioxidative systems
not evaluated in this study, by the degree of renal impairment or failure, which is
not always detectable through routine tests (Ciaramella et al. 1997 ), or by the fact
that the Sb v therapy alone may not be sufficient to determine the improvement of
the hematological indices and redox states in dogs with CanVL (Erel et al. 1999 ).
For example, in hamsters with experimental visceral leishmaniasis, the combina-
tion of antioxidants, such as -tocopherol, ascorbic acid, and flavonoids, with the

Sb v therapy resulted in a greater reduction of ROS and parasitemia (Sen et al. 2005 ),
and a greater increase in HGB concentrations and RBC half-lives as compared to
the Sb v therapy alone (Sen et al. 2004 ). It is important to note that while the levels of
SOD activity and hematological indices in dogs with CanVL were similar before
and after therapy with Sb v , but significantly different as compared to those of
control dogs, the levels of GSHPx activities before therapy were similar to that of
control dogs, and were basically reduced after Sb v therapy (Fig. 12.3 ). As previ-
ously reported during chronic experimental infections in hamsters (Sen et al. 2001 )
and cutaneous leishmaniasis in humans (Kocyigit et al. 2003 ), this finding, although
without statistical significance, may suggest a depletion of the GSHPx antioxidative
system during chronic CanVL infections in dogs. However, the lack of a statistical
significance for this finding underlines the need for increased sample size.

a