Biology Reference
In-Depth Information
Fig. 12.3
Dots
represent
individual blood GSHPx
(U/L) values.
Horizontal lines
represent mean
SD. A
¼
T
0
;
B
¼
T
60
;C
¼
CTRL
1400
1200
1000
800
600
400
200
0
A
B
C
Interestingly, pretreatment GSHPx activity is positively related with the progres-
sion of anemia, while pretreatment SOD activity is inversely related. Although
significant, the coefficient of determination
R
2
of the developed algorithm indicates
that these relationships are weak. Thus, despite a 50% variance in posttreatment
HGB concentrations, this may be explained by the variance of pretreatment SOD
and GSHPx activities, and the remaining percentage is due to other factors, such as
low iron concentrations, renal or bone marrow impairment, or other antioxidative
systems not considered here. Nevertheless, the normal distribution of the data and
the absence of colinearity between SOD and GSHPx activities support the evalua-
tion of different pretreatment antioxidative systems that may be predictive or
protective against the oxidative damage involved in anemia during CanVL. To
the authors' knowledge, this is the first study on the comparison of SOD and
GSHPx activities in dogs with CanVL before and after therapy with Sb
v
.As
previously observed during CanVL (Bildik et al.
2004
), RBCs are exposed to an
oxidative stress that could be responsible for their premature removal from circula-
tion. Our results suggest that this damage persists even after Sb
v
therapy. A study is
in progress to examine the efficacy of the combination of antioxidants with the Sb
v
therapy in dogs with CanVL.
References
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¨
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