Biology Reference
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CENTRORADIALIS) [59] were present in GDR Fragaria EST. Moreover, an
EST contig corresponding to the floral identity gene AP1 was found. The length
of the translated protein sequence of FvAP1 was 284 amino acids, being 30 amino
acids longer than the corresponding Arabidopsis sequence. However, FvAP1 EST
contig contained an unknown sequence stretch of 81 bp at nucleotide position
596-677. Putative FvAP1 showed highest overall identity (68%) with putative
AP1 from Prunus persica (Figure 3C). Moreover, the 5' sequence containing 187
amino acids (the sequence before the unknown part) was 73% identical with the
Arabidopsis AP1.
Figure 3.
Protein alignments of Fragaria flowering integrator and identity genes.
Multiple alignments
of Fragaria protein sequences of full length SOC1 (A), partial LFY (B) and full-length AP1
(C) with closest protein homologs and corresponding protein sequence of Arabidopsis thaliana. Alignments
were done by ClustalW (A, B) or T-Coffee (C) and modified by Boxshade program. F. vesca AP1 protein
sequence was translated from GDR Fragaria EST contig 4941. PTM5 = Populus tremuloides MADS5, AFL2 =
Apple FLORICAULA 2, PpAP1 = putative Prunus persica AP1.
