Biology Reference
In-Depth Information
Table 4. The list of genes belonging to autonomous and gibberellin flowering pathways.
Identification of Floral Integrator Genes in Fragaria
Sequencing of our EST collections did not reveal any homologs for the floral
integrator or identity genes such as FT, SOC1, LFY or AP1 [12,58]. A full-length
cDNA sequence of SOC1 homolog [GenBank:FJ531999] and a 713 bp 3'-end
fragment of putative LFY [GenBank:FJ532000] were isolated using PCR. Closest
protein homolog of the putative FvSOC1 was 72% identical Populus trichocarpa
MADS5, and the putative FvLFY showed highest amino acid identity (79%)
to Malus domestica FL2. Comparison to Arabidopsis showed that AtSOC1 and
AtLFY, respectively, were 66% and 75% identical with the corresponding wild
strawberry protein sequences (Figure 3A and 3B). FT homolog, instead, was
not identified in Fragaria despite of many attempts using degenerate PCR and
screening of cDNA library plaques and E.coli clones from a variety of tissues
and developmental conditions with the Arabidopsis coding sequence (K. Folta,
unpublished). However, a putative FT was found in Prunus and Malus protein
databases at NCBI. Among the other genes belonging to the same gene family,
homologs of MFT (MOTHER OF FT AND TFL1) and ATC (ARABIDOPSIS
Search WWH ::




Custom Search