Biology Reference
In-Depth Information
Gene Expression Analysis Revealed Few Differences Between
EB and SD Genotypes
We compared the expression of selected flowering time genes (Table 5) corre-
sponding to each flowering pathway in the leaf and shoot apex samples of EB and
SD genotypes in order to explore the role of different pathways. Only few of the
analysed genes were differentially expressed between the genotypes. Floral integra-
tor gene LFY was slightly up-regulated in the shoot apex samples of EB (Table 6).
Moreover, PCR expression analysis with two different primer pairs showed that
AP1 was specifically expressed in EB apices correlating with the identity of the
meristems. Among the genes from different flowering pathways, only two genes,
vernalization pathway gene ELF8 [17] and photoperiod pathway gene ELF3 [60],
were slightly differentially expressed between the genotypes (Table 6).
Table 5.
The list of PCR primers used in real-time RT-PCR.
Table 6.
The expression of selected genes in the wild strawberry.
