CHEMICAL MUTAGENESIS AS A METHOD OF BASELINE GENETIC DIVERSITY ENLARGEMENT IN PLANT BREEDING - Ecological Consequences of Increasing Crop Productivity - page 255

Electrophoregrams of 710 erytrospermum and 712 erytrospermum samples also
do not contain any new components, which are not identifi ed in Roxana variety and M
432/5 mutant (Figure 23.2).
Lines 710 and 712 belong to the same form. However, components 4 and 5, pres-
ent in line 712, is missed in line 710, which also proves the genetic difference between
them. Electrophoresis of 712 erytrospermum variety sample showed that this sample
is heterogeneous, and two biotypes were detected in it. Presence of identically lo-
cated common components of electrophoregrams, in Roxana initial variety and lines
708-712, which were chosen from nonstable 432/5 dwarf mutant, points out their
genetically similarity.
FIGURE 23.2 Electrophoretic spectra of gliadins; M 710 erytrospermum—1, 2; M 712
erytrospermum—3, 4.
Electrophoresis enables us to see practically all polymorphisms of proteins and
identify new associations of genes. In our research studies of electrophoresis of glia-
dins, we detected heterogeneity and revealed the affi nity with the initial variety of line
chosen by the phenotype criteria markers.
Gliadine electrophoregrams of number 712 erytrospermum showed that this sam-
ple is heterogenic; two biotypes were detected by the components of gliadins (Figure
23.2, lines 3, 4). Lines 710 and 712 belong to the same form—erytrospermum. How-
ever, line 710 does not have components 4,5 in line 712, which also prove differences
in their farm-valuable features. There are 19 movable components in line 712 which is
present in both biotypes. Components 1, 2, 3, 6, 7, 8, 14, and 15 are present in Roxana
variety and in line 710. Components 1, 2, 3, 7, 8, 14, and 15 were common for line 712
and Roxana variety. Presence of placement common components of gliadins 1, 2, 3, 7,
14, and 15 in Roxana initial variety and lines 708, 710, and 712 chosen from nonstable
432/5 dwarf mutant shows their genetic affi nity with Roxana variety.
The research results prove that electrophoresis method of gliadins in polyacryl-
amide gel can be used for identifi cation of winter wheat mutant forms.
23.4 CONCLUSIONS