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quence of gene nucleotide structure. That is why proteins are markers of both single
structural genes and their associations. Allele variants of proteins are usually inherited
codominantly; their content is not predetermined with growing conditions and is de-
tected comparatively easily by electrophoresis method [31].
We started the research studies on identifi cation by electrophoretic spectrum of
gliadins in 2001 and they have been carried over until now. Collection of mutants
used as a baseline was set up in the previous years at the Department of Genetics,
Breeding and Seed production of Bila Tserkva National Agrarian University. In 1998,
S. Vasilkivskiy by the method of chemical mutagenesis bred from Czech Republic
Roxana variety diazoacetylbutane 0.025 percent concentration induced and selected
432/5-dwarf mutant, which was not stable. The lines, which gave rise to new ones,
were bred in its generations.
The 432/5 mutant belongs to real dwarf species by its morphological characteris-
tics. The result of electrophoresis (Figure 23.1) results in conclusion about heterogene-
ity by number, intensity, and electrophoretic activity of compounds.
The M 432/5 contains no new components, which are missing in Roxana variety.
FIGURE 23.1
Electrophoretic spectra of winter wheat gliadins for 432/5 mutant.
In their practical work with mutants, research studies reject the forms, which in
their fourth-fi fth generations give splitting with deviations in their quantity correla-
tions from the theoretically expected. We managed to work out a way of using new
source of genetic changeability and to offer the method of working with nonstable
mutants attributed to years of research at the Department of Genetics, Breeding and
Seed Production of Bila Tserkva National Agrarian University [32, 33, 34].
In 1999-2005 in posterity of M 432/5 dwarf erytrospermum form, plants were in-
dividually chosen, different in their anatomy morphological and agricultural features,
one of them gave rise to a new 710 erytrospermum line, another—for 712 erytrosper-
mum line.
Presence of the same components in these lines points on their affi nity, affi nity
with 432/5 mutant and with Roxana initial variety. Absence of some components
proves their difference in both genetic and morphological levels.
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