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The registration of light diffusion by dilute suspension of EAK cell, of thymo-
cytes, of lymphocytes and platelets, was held by the method Cornet [23], modi¿ ed by
Zinchenko [21] at a right angle under the wavelength 510 nm with aid of À uorescent
spectrophotometer “MPF-44ȼ” PerkinElmer.
19.3 DISCUSSION AND RESULTS
The using of light scattering method allowed us to investigate the overall cellular
answers under the Melafen additions without any artificial messengers. Thanks to pre-
sented method, we tested the action of Melafen to the animal cells and its components
under a wide range of concentrations.
The three cellular objects were used, as the dilute cells cellular suspension: EAC
cells - transformed cells with uncontrolled growth, and normal cells thymocytes and
lymphocytes (the white fraction of blood without the platelets and erythrocytes).
First EAC cells, as a good model of cells with the complete cellular transduction
system. The active P2Y purinoreceptors are presented at the cellular plasmalemma
surface at the 7-8 days of carcinoma growth [20]. Thus the ATP or ADP additions initi-
ated the Ca
2+
signal transduction (Figure 2). The ATP is the ¿ rst messenger that deals
with the extracellular signal transductions pathways. The ATP is released to the extra-
cellular space. At least two subtypes of receptors for extracellular ATP are currently
known: the G-protein-coupled P2Y receptors, which are methabotrophic receptors,
and the ATP-gated cation channels classi¿ ed as P2X receptors (and its subspecy - P2Z
receptors). The EAC cells gave a typical cellular response to a signal (ATP-addition).
It sent the signal from the cell surface to inside the endoplasmic reticulum (ER) InsP
3
-
receptor, and backward to the CRAC at the cell surface.
FIGURE 2
The total scheme of P2Y, P2X, and P2z -related system of signal transduction
(scheme was modified from Alekseeva O.M. 2010).
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