Molecular Biology

MonoADP-ribosylation is a post-translational modification, catalyzed by ADP-ribosyltransferase (ADPRT), that transfers the ADP-ribose moiety from NAD+ to a specific amino acid residue of target protein and releases the nicotinamide moiety. To date, four amino acid-specific ADPRT have been reported, specific for arginine, cysteine, diphthamide (a modified form of histidine), and asparagine residues. These transferases were […]

1. The Basic Principle Affinity chromatography (AC) is a general chromatographic method for the selective extraction and purification of biological macromolecules on the basis of their biorecognition (1-3). The method makes use of the specific physiological affinity between a desired macromolecule (M) and one of its physiological ligands (L). The ligand, or its analogue (L’), […]

Affinity labeling is a strategy to modify chemically an amino acid residue within a specific ligand-binding site of an enzyme, either at the active site or at a regulatory, allosteric site. In this approach, a reagent is designed that resembles structurally the natural ligand of the enzyme but features in addition a functional group capable […]

A key requirement of combinatorial library approaches is that desirable molecules must be segregated from the remaining library population. Segregation is most often accomplished by affinity partitioning, in which an immobilized target is used to capture interacting molecules from a solution-phase library. Target proteins can be immobilized either using antibodies bound to Staphylococcus protein A, […]