Biomedical Engineering Reference
In-Depth Information
Fig. 13.7 Detection of nerve
agent hydrolytes. (a) Side
view of the (M113F/K147N)
7
pore; and (b) View into the
(M113F/K147N)
7
pore from
the
cis
side of the lipid
bilayer, highlighting positions
113 and 147, and the lodged
b
CD molecule. (c) Structures
of PMPA and CMPA. Typical
single channel current
recording traces: (d) Without
PMPA/CMPA; (e)2
a
c
O
CH
3
H
C
H
3
C
P
P
O
O
C
C
C
C
CH
3
cis
OH
CH
3
CH
3
trans
PMPA
b
O
H
3
C
P
O
m
M
PMPA; and (f)2
M CMPA.
The experiments were
performed at
m
OH
80 mV in 1 M
NaCl and 10 mM Tris-HCl
(pH 7.5), and in the presence
of 40
CMPA
d
0
CD. Figure
reprinted with permission
from [
15
], Copyright 2009
Elsevier B.V.
m
M
b
−
20
−
40
−
60
0
1000
2000
3000
4000
e
Time (ms)
0
−
20
−
40
−
60
0
1000
2000
3000
4000
f
Time (ms)
0
−
20
40
−
−
60
0
1000
2000
3000
4000
Time (ms)
with a boromycin host can be employed for the analysis of organophosphorus
chemical agents. For example, a host beta-cyclodextrin (
b
CD) molecule can be
lodged in the lumen of the channel of an engineered
HL (M113F/K147N)
7
pore
(as depicted in Fig.
13.7a, b
) as a molecular adapter to detect pinacolyl
methylphosphonic acid (PMPA) and cyclohexyl methylphosphonic acid (CMPA,
Fig.
13.7c
), the hydrolyzed products of the nerve agents, GD and GF respectively
[
15
]. Note that in the two nanopore sensing systems (i.e., detection of liquid
explosives and nerve agent hydrolytes), the host compounds boromycin and
a
b
CD
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