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Fig. 4.12 One way-traffic in DNA translocation through a connector channel in a lipid bilayer
verified by switching the voltage polarity: (a) No DNA in either chamber; (b-d) The presence of
DNA in both chambers where a single connector was inserted. Figures reproduced with permis-
sions from: Ref. [
44
],
American Chemical Society
#
when dsDNA translocation was observed under negative potential, switching
the voltage to positive potential resulted in no dsDNA translocation (Fig.
4.12d
).
The same results were also confirmed under different voltages.
4.6.3 One-Way Traffic of dsDNA Was Probed by Quantification
of DNA Translocation Frequency in the Presence
of Multiple Channels
Since dsDNA only traverses through connector channels from one direction, the
DNA translocation rate would therefore be affected by different arrangements
of connector in the BLM in presence of multiple channels, regardless of the DNA
concentration. Accordingly, the experiments were carried out under a constant
holding potential of
75 mV with varying concentrations of dsDNA (Fig.
4.13
).
The current traces with multiple connector insertions reflect the change in the
frequency of DNA translocation events (represented in the histograms in
Fig.
4.13
). When one additional connector was inserted with an appropriate orien-
tation to allow DNA to pass, the frequency of DNA translocation increased
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