Biomedical Engineering Reference
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Fig. 4.11 One way-traffic in DNA translocation through a single connector channel in a lipid
bilayer under a ramping potential from
100 mV to +100 mV (2.2 mV/s): (a) Without DNA; (b, c)
DNA added in both chambers with a single connector. Figures reproduced with permissions from:
Ref. [
44
],
American Chemical Society
#
4.6.2 One-Way Traffic of dsDNA Probed by Applying a Constant
Holding Potential and by Switching the Voltage Polarity
to a Membrane with a Single Channel
Under a constant potential, switching of voltage polarity revealed that the connector
channel allowed only a unidirectional translocation of dsDNA (Fig.
4.12
). DNA
was pre-mixed in both chambers at equal concentrations. The polarity at which
DNA translocation was observed was dependent upon the orientation of the connector
in the BLM. Figure
4.12a
shows the control experiment in absence of DNA. For
example, when there was no DNA translocation under positive potential, switching
the voltage to negative potential resulted in dsDNA translocation (Fig.
4.12b
). Con-
versely, when there was no DNA translocation under negative potential, switching the
voltage to positive potential resulted in dsDNA translocation (Fig.
4.12c
). Similarly,
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