Biology Reference
In-Depth Information
Table 8.2
Genomics of Chamaedorea and Euterpe
Genus and System
Methods and Conclusion
Reference
Chamaedorea—
AFLP
249 samples (127—
C. tepejilote
Lieb.; 122—
C.
alternans
Wendl.) of 44 accessions in each using
primer combinations EcoRI -GAG TGC GTA CCA
ATT C- & MseI; GAT GAG TCC TGA GTA A;
2 species are genetically different; 5 of 45 loci
display species specific fragments
[6]
Chamaedorea—
PRK, RPB2
Sequence data of PRK (700 bp) and RPB2 (800 bp)
from 63 species from 8 subgenera,
Chamaedorea
(57 species) confirmed as monophyletic genus; a
6 bp C-rich repeat, poly-T (22 bp) and poly-C (62 bp)
regions of RPB2 interfered PCR amplification in
7 species; -A homopolymer (31 bp) in PRK
prevented alignment across species.
[7]
Chamaedorea tribe-
PRK RPB2,
trnL-trnF,
rps
16,
ndhF, matK, and
trnD-trnT
40 species of tribe Chamaedoreeae—Hyophorbe,
Gaussia, Synechanthus, and Wendlandiella-
all species; Chamaedorea—27 species; PRK
(531-638 bp), RPB2 (666-828 bp), and 5 plastid
regions (trnL-trnF,
rps
16, ndhF, matK, and trnD-
trnT); biography—northern distribution; 5 loci
(Plastid regions—127 informative sites; RPB2-
141 informative sites) good for phylogenetic
classification
[8]
Chamaedorea—SSR
138 individuals of
C. ernesti-augusti
in 8 locations
of Belize, Central America by 9 SSR loci; high
degree of inbreeding, linkage disequilibrium at few
locations
[9]
Chamaedorea—
Allozyme
126 individuals of
C. tepejilote
(59 alleles) and
183 of
C. elatior
(53 alleles) palms; higher spatial
genetic structure in
C. tepejilote
than
C. elatior
;
because of diverse mix of family patches with
many individuals; Hence (high degree of within
population) genetic diversity
[10]
Euterpe—Allozyme
70-100 individuals of
E. edulis
in 6 plots at Santa
Catarina (Brazil); 10 enzyme systems gave 11
ploymorphic loci-peroxidase, 6-phosphogluconate
dehydrogenase and glucose-6-phosphate
dehydrogenase gave specific alleles linked to
recruitment process
[11]
Euterpe—SSR
66 individuals in natural population at Cerrado gallery
of Atlantic using 18 polymorphic SSRs helpful in
gene flow analysis & paternity testing
[12]
Euterpe—AFLP
150 plants of 11 populations analyzed by 429 AFLP
markers reveal moderate within population variation
(57%); geographical distance and genetic distance
are positively correlated in these populations
[13]