Biology Reference
In-Depth Information
Fig. 2. (
a
) LASCA images before (
left
) and after (
right
) cerebrovascular reactivity testing through acetazolamide application
in a C57/BL6 mouse 6 h after experimental SAH. The speckle contrast is displayed in a color-coded real-time matrix. Tissue
perfusion in the arbitrary unit fl ux can be referenced to the legend on the
right-hand side
. The fl ux values are calculated in
the left and right hemispheric ROI and plotted over time. (
b
) Real-time CBF-fl ux in two regions of interest. The
straight
and
dashed
line refer to the mean fl ux value within the rectangular left- and right-hemispheric ROIs before and after acetazo-
lamide is given to increase cortical CBF. The CBF-fl ux values are plotted over time (
x
-axis).
2.2. Instruments
and Materials for
Surgical Preparation
To surgically expose the scalp, the following instruments are
recommended (Fig.
3
):
Small surgical scissors and microscissors
●
2.2.1. Instruments
Forceps (anatomical and microsurgical)
●
Needle driver
●
Surgical microscope (optional)
●
Fur trimmer/razor
●
In addition, the following materials are required:
2.2.2. Materials
Portable surface/pad for mobile positioning of the mouse
●
Temperature-controlled heating pad
●
4× Silk sutures (6/0)
●
1× Nylon suture (6/0)
●
Cotton swabs
●
Sterile saline irrigation
●
Paraffi n oil
●
Alcoholic disinfectant
●
Search WWH ::
Custom Search