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Fig. 4. (
a
) Skin incision from glabella to cervical vertebra is placed. (
b
) Occipital muscles
are cut and refl ected bilaterally. Craniotomy is started from atlanto-occipital junction
(
open arrowhead
). (
c
) Brain and upper spinal cord is exposed after craniotomy. (
d
) Skull
base after removal of brain.
anesthetic is confi rmed. This procedure should be selected,
when your institute does not allow any agony as a way of
taking specimens on animal. In this method, thoracotomy
and cannulation for perfusion fi xation should be immedi-
ately performed, since the degeneration of the tissue is
induced after heart arrest. And heparin should be injected
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