Application of Zymographic Methods to Study Matrix Enzymes Following Traumatic Brain Injury - Animal Models of Acute Neurological Injuries: Injury and Mechanistic Assessments - page 181

Closed head injury
Weight drop
Juvenile rat

Gelatin zymography Cingulate, parietal cortex

Thalamus
Frozen/lysis buffer
Brij 35, Triton X-100
Gelatin sepharose 4B
Affi nity purifi cation

Sifringer et al. 2007 ( 9 )

Blade lesion of cortex Gelatin zymography Frontal/parietal cortex

Brainstem, cerebellum
Total protein extracts

Yhamaguchi et al. 2007 ( 10 )

Entorhinal lesion
Fluid percussion injury

Fluorometric assay Hippocampus

Lysis buffer/Tris

Kim et al. 2005 ( 11 )

Fluid percussion injury
Hypothermia

Gelatin zymography
In situ zymography

Frozen/cortex, hippocampus,
thalamus
Lysis buffer, Tris/SDS

Truettner et al. 2005 ( 12 )

Subarachnoid hemorrhage In situ zymography Striatum Sheba et al. 2004 ( 13 )

Controlled cortical impact Gelatin zymography Whole brain/cortex

Fresh frozen/lysis buffer
Lysis buffer/Nonidet P-40, SDS

Mori et al. 2002 ( 14 )

Scratch in vitro injury Gelatin zymography Culture media supernatant

Lysis buffer/SDS

Wang et al. 2002 ( 15 )

Entorhinal lesion
Fluid percussion injury

Gelatin zymography Hippocampus

Lysis buffer/T-PER

Phillips and Reeves 2001 ( 16 )

Aspiration corticectomy Gelatin zymography Frozen/perilesional cortex

Lysis buffer/TritonX-100

Vecil et al. 2000 ( 17 )

Controlled cortical impact Casein zymography Cortex

Lysis buffer/cytosolic extracts

Zhao et al. 1998 ( 18 )

Listed are a sampling of published studies which have assayed metalloproteinase activity following brain insults. The majority utilize gelatin zymography.
Notably, a wide range of models have been examined and choice of sample preparation may vary considerably. Extraction from fresh tissue is indicated simply
by the area sampled while protocols which store dissected tissue prior to processing are noted as frozen preps