Application of Zymographic Methods to Study Matrix Enzymes Following Traumatic Brain Injury - Animal Models of Acute Neurological Injuries: Injury and Mechanistic Assessments - page 180

Table 1
Current approaches to matrix enzyme assay in CNS trauma

Model Method Sample/preparation References

Impact acceleration Gelatin zymography Cortex

Frozen/lysis buffer
Nonidet P-40

Ding et al. 2009 ( 1 )

Clinical population
Severe TBI

Gelatin zymography
Fluorometric assay

Plasma/CSF
CSF protein extracts
Lysis buffer/Tris, SDS

Grossetete et al. 2009 ( 2 )

Intracerebral hemorrhage Gelatin zymography Frozen/cortex

Lysis buffer/TritonX-100
Gelatin sepharose 4B
Affi nity purifi cation

Grossetete and Rosenberg 2009 ( 3 )

Closed head injury
Weight drop

Gelatin zymography Cortical punch

Lysis buffer Brij 35
Triton X-100
Gelatin sepharose 4B
Affi nity purifi cation

Homsi et al. 2009 ( 4 )

Hypoxia/ischemia
Cortical development

Gelatin zymography Cortical hemispheres

Frozen/lysis buffer
Nonidet P-40

Ranasinghe et al. 2009 ( 5 )

Peripheral thermal injury Gelatin zymography Whole brain

Total protein extracts

Reyes et al. 2009 ( 6 )

Clinical population
Moderate/severe TBI

Gelatin zymography Plasma

Frozen/lysis buffer
Nonidet P-40

Vilalta et al. 2008 ( 7 )

Spinal cord injury Gelatin zymography

In situ zymography

Spinal cord
Frozen/Brij 35 lysis buffer
Gelatin sepharose 4B
Affi nity purifi cation

Yu et al. 2008 ( 8 )