Chemistry Reference
In-Depth Information
Table 4.5
(continued)
Enzyme
Additive
Comment
Activity
a
Ref.
Lipase (Pseudo-
monas cepacia)
PA H
Good levels of activity were maintained
compared to free enzyme. Activity was
enhanced further (up to 3 times) by using
alkyl-alkoxysilanes. Improved stability and
good storage potential was seen
51
[
80
]
Acetylcholines-
terase;
Glucose oxidase;
β-Galactosidase
PEI
Maximum rates (and therefore activity)
of immobilised system was less than free
enzyme system. Immobilised enzyme also
needed higher substrate concentrations
than free enzyme
40
b
[
87
]
Adenosine
deaminase;
Nucleoside
phosphorylase;
Xanthine
oxidase
PLL
The immobilised enzymes were used
to produce biosensors. The biosensors
produced performed well, with high per-
formances, suggesting immobilisation had
little detrimental effect on the enzymes.
The procedure was also adaptable for other
enzymes
N/S
[
83
]
β-glucoronidase Prot-
amine
Activity of immobilised system was less
than free enzyme system. Leaching was
negligible, an improvement in stability was
seen, and the material had excellent reuse
and storage potential
20-30
b
[
86
]
Glucose oxidase;
Horseradish
peroxidase
PAMAM
Good levels of glucose oxidase activity
were retained during immobilisation in
water (better than horseradish peroxidase).
It showed very little leaching and good
storage potential. Horseradish peroxidase
performed better that glucose oxidase
when immobilised in buffer
4-54
13-39
[
82
]
D-amino acid
oxidase
PA H
High levels of enzyme activity were main-
tained upon immobilisation. An improve-
ment in stability was seen, compared to
free enzyme
96
[
81
]
Papain
PLL-
b
-
PLG
c
The immobilised enzyme retained good
activity and demonstrated enhanced pH
and thermal stability, as well as good
reuse potential. With this approach, the
papain was first entrapped in the block co-
polypeptide vesicles, and added to a silica
precursor to initiate silica formation
[
88
]
∼ 40
b
Invertase
PEHA
First report presenting enzyme immobilisa-
tion using flow chemistry useful for con-
tinuous production. Enzyme was observed
to be active upon immobilisations
N/S
[
91
]
N/S
not stated
a
Activity % cf. Free Enzyme
b
Activity estimated from kinetic parameters
c
Poly-L-lysine-
b
-poly-L-glycine copolymer
