Chemistry Reference
In-Depth Information
Table 4.5
A list of
enzymes immobilised using bioinspired silica
Enzyme
Additive
Comment
Activity
a
Ref.
Butyrylcholines-
terase
R5
The immobilised enzyme appeared to
retain virtually all of its activity. An
improvement in stability was also observed
[
74
]
> 90
Catalase;
Horseradish
peroxidase
R5
Both enzymes showed good stability,
storage potential and activity similar to the
free enzyme
90
90
[
76
]
β-Galactosidase R5
Enzyme immobilised in silica nano-
spheres were attached to a silicon support,
with very little loss of activity detected.
Biocatalysts showed very good storage
potential
[
2
,
45
]
∼ 90
Hydroxylamino-
benzene mutase
(HABM);
Soybean peroxi-
dase (SBP)
R5
The performance of immobilised enzymes
was successfully tested in a microfluidic
device. Greater levels of activity were
maintained for SBP than HABM. The
immobilised materials exhibited good
reuse potential
45-65
65-85
[
89
]
Lipase;
Glucose
isomerase
PEI
Immobilised lipase retained very low
levels of activity, while glucose isomerise
was reasonably active. Poor lipase activity
was thought to be due to PEI creating a
strongly hydrophilic environment, making
substrate access difficult. Lipase did, how-
ever, showed improved thermal stability
and a wider pH optimum
10
40
[
2
,
77
]
Horseradish
peroxidase
PEI
PAMAM
Immobilised enzymes were used for
biosensor applications. PEI appeared
most efficient for silica formation and the
immobilised enzyme was effective for use
in a biosensor
N/S
[
79
]
Carboxylesterase
Lyso-
zyme
R5
PEI
Both lysozyme and R5 proved successful;
however, immobilisation was unsuccessful
when PEI was used
N/S
[
78
]
Hexose oxidase
PEI
Enzyme was tested for potential use as
enzyme based anti-fouling coatings.
Immobilised enzyme showed improved
stability, including in an artificial seawater
environment, as well as good long term
properties
6-13
b
[
85
]
Luciferase
Cyste-
amine
Ethanol-
amine
Higher levels of activity were maintained
using cysteamine, rather than ethanol-
amine. It was therefore deduced that this
was due to cysteamine having better cata-
lytic properties for silica formation
87
17
[
90
]
