Chemistry Reference
In-Depth Information
a
b
125
125
100
100
75
75
12
11
50
12
Light controls
11
Light controls
50
25
25
0
0
0.0
0.5
1.0
1.5
2.0
2.5
0.01
0.1
1
10
Light Dose (J/cm
2
)
Drug Concentration (
m
M)
Fig. 4 In vitro photosensitizing efficacy (MTT assay) of Ni(II) purpurinimide-cyanine dye
conjugate 11 and the corresponding non-metallated analog 12;(a) PDT efficacy at a fixed
concentration of the photosensitizers, but the cells were exposed to variable light doses, (b)
PDT efficacy at a fixed light dose, but at variable concentrations. On exposing the cells with
light without incubating with photosensitizers 11 and 12, no dark toxicity (cell kill) was observed.
The cells were incubated for 24 h before the light exposure
Scheme 2 Synthesis of metallated [Ni(II)] and free-base mesopurpurinimide-cyanine dye
conjugates
