In vitro Antioxidant Analysis and the DNA Damage Protective Activity of Leaf Extract of the Excoecaria agallocha Linn Mangrove Plant - Agricultural Chemistry - page 164

Lane E: DNA + Fenton's reagent + E. agallocha. Linn hexane extract, Lane F: DNA + Fenton's reagent + E.
agallocha. Linn water extract (100g/ml), Lane G: DNA + Fenton's reagent + E. agallocha Linn. crude
methanol extract (100g/ml) and Lane H: DNA + Fenton's reagent + quercetin (50 M), Lane I: DNA +
Fenton's reagent + Catalase (5 units).
6. Conclusion
In conclusion, the result obtained in the present study shows that the methanolic extract of
E. agallocha contains a number of antioxidant compounds that can effectively scavenge ROS.
Antioxidant properties of botanical extracts should be assessed in an array of model systems
using several different indices because the effectiveness of such antioxidant material is
largely dependent upon the chemical and physical properties of the system to which they
are added and a single analytical protocol adopted to monitor lipid oxidation may not be
sufficient to make a valid judgement. Hence, it may be concluded that the strong radical
scavenging activity and oxidative DNA damage preventive activity of E. agallocha Linn may
be correlated with its rich content of flavonoids.
Author details
C. Asha Poorna, M.S. Resmi and E.V. Soniya *
Plant Molecular Biology, Rajiv Gandhi Centre for Biotechnology, Government of India, Trivandrum,
India
7. References
[1] Alma, M.H., Mavi, A., Yildirim, A., Digrak, M., Hirata, T. Screening chemical
composition and antioxidant and antimicrobial activities of the essential oils from
Origanum syriacum L. growing in Turkey. Biol. Pharm. Bull . 2003, 26, pp 1725-1729.
[2] Verma A.R., Vijayakumar A, Rao C.V., Mathela C.S. In vitro and in vivo antioxidant
properties and DNA damage protective activity of green fruit of Ficus glomerata , Food
and Chemical Toxicology . 2010, 48, pp 704-709.
[3] Yin, B.W., Shen, L.R., Zhang, M.L., Zhao, L., Wang, Y.L., Huo, C.H., Shi, Q.W. Chemical
Constituents of Plants from the Genus Excoecaria. Chemistry and Biodiversity 2008, 5, pp
2356- 2371.
[4] Murray, P.R., Baron, E.J., Pfaller, M.A., Tenover, F,C., Yolke, R,H. Manual of Clinical
Microbiology, 6 th ed. ASM, Washington, DC. Service, R.F, Antibiotics that resist
resistance. Science . 1995, 270, pp 724-727.
[5] Brand, W.W., Cuvelier, M.E., Berset, C. Use of free radical method to evaluate
antioxidant activity. Lebensmittel- Wissenschaft und Technologie . 1995, 28, pp. 25-30.
[6] Oyaizu, M. 1986, Studies on the products of browning reaction prepared from glucose
amine. Japan Journal of Nutrition , 1986, 44. pp. 307- 315.
* Corresponding Author