Biomedical Engineering Reference
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Fig. 9.7 Photo images of radioactive signals. ( a ) whole brain in-situ hybridization images under
silent and singing conditions, using X-ray fi lm images under bright light (top panels; black = mRNA
signal), inverted X-ray fi lm images (2nd low; white = mRNA signals), silver grain dipped-cresyl
violet stained images under regular bright light (3rd low; black = mRNA signals, blue/purple =
cresyl violet stained cells), and silver grain dipped-cresyl violet stained images under polarized light
in dark fi eld (4th low; white = mRNA signals, purple/red = cresyl violet stained cells). Scale bar =
2mm. ( b ) Higher magnifi cation of silver grain dipped-cresyl violet stained images under regular
bright light. Developed silver grains are observed as black dots. Scale bars = 200µm
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