Design of Mobile Supramolecular Biointerfaces for Regulation of Biological Responses - Biointerfaces: Where Material Meets Biology - page 355

d n 3 r 4 n g | 3

Figure 12.9 Morphology of adhering platelets and the number of adhering platelets
plotted with g-chain exposure of adsorbed fibrinogen.
Reproduced/adapted from ref. 28.

normally increased in proportion to the amount of adsorbed fibrinogen.
However, the mPRX surface showed different tendency with those of the
other materials surfaces. Namely, it is considered that eliminating con-
formational change of adsorbed proteins including fibrinogen on the dy-
namic mPRX surface can prevent platelet adhesion, in spite of significant
amount of adsorbed proteins.

.

12.4.3 Adsorption and Adhesion Behavior of Fibronectin and
Human Umbilical Vein Endothelial Cell on the
Dynamic Surfaces
Adhesion behavior of the different type of cell on the dynamic PRX surface
was explored using human umbilical vein endothelial cells (HUVECs). Being
different to the platelet responses promoted adhesion of endothelial cells on
the biomaterial surfaces is preferable in designing blood-contacting bio-
materials for the regeneration of the damaged tissues. 41 However, non-
specifically adsorbed surface protein, which is required for the adhesion of
HUVECs, also triggers undesirable biological responses such platelet ad-
hesion and activation. This paradoxical problem of the surface protein has
been a fundamental barrier to developing ideal biomaterials.
Fibronectin, a major cell adhesive protein in serum, exposes the RGD
and synergetic pentapeptide sequence (PHSRN) when it adsorbs on