Biomedical Engineering Reference
In-Depth Information
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Figure 12.8
Schematic explanation of properties of the dynamic PRX interfaces
contacting with protein molecules as preserving their conformation.
12.4.2 Platelet Responses on the Dynamic Surfaces
Previous studies on adsorbed fibrinogen on polymer surfaces demonstrated
that C-terminus of g-chain presenting dodecapeptide sequence in fibrinogen
is essential to induce platelet-fibrinogen interaction.
37
Therefore, how
well suppress the presentation of platelet GPIIb/IIIa binding site in the
C-terminus g-chain could be a key to developing 'bio-inert' blood-contacting
materials. To estimate the effect of adsorbed plasma proteins on the cellular
responses, a platelet adhesion test was carried out by using human platelet-
rich-plasma (PRP). Figure 12.9 shows the result of platelet adhesion on the
prepared polymer surfaces. Obviously, the Cell Desk
t
surface induced a
large amount of platelet adhesion on its surface, suggesting that the platelet
adhesion is derived from significant conformational change of adsorbed
proteins including fibrinogen as confirmed by ELISA. In contrast, the mPRX
surface showed eliminated platelet adhesion even though it induced sig-
nificant amount of fibrinogen adsorption. Figure 12.9 also shows the
quantitative result of adhering platelets along with the relative amount of
C-terminus g-chain binding antibody. Obviously, an almost straightforward
relationship between the number of adhering platelets and the relative
amount of exposed g-chain was observed. Namely, the platelet adhesion was
increased with the extent of exposing C-terminus g-chain of adsorbed fi-
brinogen on the surface, and this tendency is consistent with previous re-
port.
38-40
.
It has been already reported that amount of platelet adhesion is
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