Chemistry Reference
In-Depth Information
Fig. 2.2
A patch clamp amplifier is integrated to a microfluidics station on inverted microscope
to make electrical recordings in microfluidic devices
solution can be corrected for out immediately. At the inflection points of the sine
wave, where
V
m
(
t
)
=
0, only
I
C
is measured and by using the derivative of Eq.
2.1
we obtain:
I
C
=
C
m
dVm
(
t
)/
dt
=
C
m
ω
V
0
cos
(ω
t
)
(2.2)
From knowledge of the experimental parameters,
ω
,
I
C
and
V
0
, the membrane
capacitance
C
m
is calculated.
2.3 Results
It has been recognized long ago that for micro-fluidics to unfold its full power, it is
advantageous to use two immiscible liquids as theworkingmedium instead of a single
liquid phase [
14
]. Not only can isolated droplets immersed in a continuous liquid
be used as compartments for controlled reactions [
15
], but the complex interplay
of the liquid/liquid interface with the channel geometry and hydrodynamic pressure
gradients gives rise to an abundance of effects which can be harnessed into the
engineering of functional devices. So far, this has been mainly limited to the use of
isolated aqueous droplets in an oily continuous phase, as containers for chemical,
biochemical, or biological processes. In these devices, the droplets are transported
