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It was first reported by Kim and Rebeiz (
1996
). Later on Kolossov, solubilized the
enzyme fromBarley etiochloroplasts (Kolossov and Rebeiz
2010
). The involvement
2.10.2.2 Discovery of DV Mg-Proto Monoester Vinyl Reductase
4-vinyl MPE reductase (VMPER) catalyzes the reduction of the vinyl group of
MPE to ethyl, at position 4 of the Macrocycle (Fig.
2.7
, 2c, 2d).
In 1973, Ellsworth and Hsing reported the reduction of DV MPE to MV MPE
by a soluble NADH-dependent enzyme in etiolated wheat homogenates (Ellsworth
and Hsing
1973
). However to our knowledge, no one, including ourselves has been
able to duplicate this work.
The unambiguous detection of 4VMPER was first reported and solubilized by
Kolossov and Rebeiz (
2010
). The involvement of VMPER in Chl biosynthesis is
2.10.2.3 Discovery of DV Pchlide a Vinyl Reductase
4-vinyl Pchlide reductase (VPideR) catalyzes the reduction of the vinyl group of
DV Pchlide
a
to ethyl, at position 4 of the Macrocycle (Fig.
2.7
, 3a, 3b).
The detection of VPideR was first reported by Tripathy and Rebeiz (
1988
).
It was solubilized by Kolossov and Rebeiz (
2010
). The VPideR gene has been
detected in green sulfur bacteria (Gomez Maqueo Chew and Bryant
2007
). The
2.10.2.4 Discovery of DV Chlorophyllide a Vinyl Reductase
4-vinyl Chlide reductase (VChlideR) catalyzes the reduction of the vinyl group of
DV Chlide
a
to ethyl, at position 4 of the Macrocycle (Fig.
2.7
, 4a, 4b).
VChlideR was the first vinyl reductase to be detected. I was first detected by
Duggan and Rebeiz in 1982 and shown to be a very fast acting enzyme (Duggan and
Rebeiz
1982a
,
b
). It was shown to have an absolute requirement for NADPH,
and was solubilized and partially purified by Parham and Rebeiz (
1992
,
1995
).
It was studied further by Kolossov and Rebeiz (
2010
). The VChlideR gene
has been first detected in
Arabidopsis
(Nagata et al.
2005
). The involvement of
2.10.2.5 Discovery of DV Chlorophyll a Vinyl Reductase
4-vinyl Chl
a
reductase (VChlaR) catalyzes the reduction of the vinyl group of DV
Chl
a
to ethyl, at position 4 of the Macrocycle (Fig.
2.7
, 4c, 4d).
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