Some Major Steps in the Understanding of the Chemistry and Biochemistry of Chl - Chlorophyll Biosynthesis and Technological Applications - page 15

1.2.36 Rebeiz and Castelfranco, 1971b
In 1971, Rebeiz and Castelfranco also reported the total biosynthesis of 14 C-Chl
a and b from 14 C-ALA in an in organello system from higher plants (Rebeiz and
Castelfranco 1971b ).
1.2.37 Gorchein, 1972
In 1972, Gorchein, demonstrated the conversion of exogenous Proto to Mpe in
R. Spheroides, and the ATP requirement of the process (Gorchein 1972 ).
1.2.38 Griffiths, 1974
In 1974, Griffith, demonstrated that NADPH is the hydrogen donor for the photo-
reduction of Pchlide a to Chlide a (Griffiths 1974 ).
1.2.39 Beale and Castelfranco
In 1974, Beale and Castelfranco demonstrated that in green plants ALA is formed
from glutamic acid. That led to the formulation of the C-5 biosynthetic pathway of
ALA formation in green plants (Beale and Castelfranco 1974 ).
1.2.40 Rebeiz, 1975
In 1975, Rebeiz et al., illustrated the usefulness of Fluorescence spectroscopy by
detecting the formation of several Mg-porphyrins during greening of etiolated
tissues, and described their spectrofluorometric properties (Rebeiz et al. 1975b ).
1.2.41 Poulson and Polglase, 1975
In 1975, Poulson and Polglase demonstrated that protoporphyrinogen IX oxidase
(Protox for short) catalyzes the conversion of Protogen IX to Proto (Poulson and
Polglase 1975 ).