Development of Analytical and Preparatory Techniques - Chlorophyll Biosynthesis and Technological Applications - page 74

and,

E420 Z 592
570

E420 Z 620
592

k 2 ¼ Zn-Proto ð f ðλÞ d λÞ= Zn-Proto ð f ðλÞ d λÞ

E420 R 620
592

the
fluorescence integral of the Mg-Porphyrins between 592 and 620 nm which was
elicited by a 420 nm excitation of the hexane-extracted acetone solution. Zn-Proto
ð

According to the above terminology: Mg-Porphyrins ð f ðλÞ d λÞ¼

E420 R 620
592

the fluorescence integral of Zn-Proto between 592 and
620 nm which was elicited by a 420 nm excitation of the hexane-extracted acetone
solution. Zn-Proto ð E420 R 592
570

f ðλÞ d λÞ¼

λÞ¼ the fluorescence integral of Zn-Proto
between 570 and 592 nm which was elicited by a 420 nm excitation of the
hexane-extracted acetone solution, etc. By solving Eqs. ( 3.27 ) and ( 3.28 ) for Mg-
Porphyrins

f ðλÞ d

E420 R 620
592

ð f ðλÞ d λÞ , the following equation is obtained:

Mg-Porphyrins E420 Z 620
592

E420 Z 620
592

f ðλÞ d λ ¼ f ðλÞ d λÞ

k 2 1

E420 Z 592
570

ð f ðλÞ d λ= = K

(3.30)

Where:

K ¼ 1 k 1 = k e (3.31)

k 1 and k 2 were calculated according to Eq. ( 3.29 ). The fluorescence integrals used in
Eq. ( 3.29 ) were determined by planimetry from the emission spectra of standard
MPE-equivalent and Zn-Prot in hexane-extracted acetone as described in (Smith
and Rebeiz 1977a ).The value of k 1 was 0.12 and was the mean of 51 different
determinations performed on various concentrations of standard Mg-Porphyrins.
The value of k 2 was 1.22 and was the mean of 19 different determinations
performed on various concentrations of Zn-Proto. The value of K, calculated
according to Eq. ( 3.31 ) was 0.90 (Smith and Rebeiz 1977a ). By substituting the
values for k 2 and K in Eq. ( 3.30 ) the following equation was obtained:

E420 Z 620
592

E420 Z 620
592

Mg-Porphyrins ð f ðλÞ d λÞ¼ð 1 : 11 Þð f ðλÞ d λÞ

E420 Z 592
570

ð : Þð f ðλÞ d λÞ

0 91 (3.32)