Biology Reference
In-Depth Information
3.3.1 Calculation of the Fluorescence Integral Between
592 and 620 nm Which Is Contributed Solely by
Mg-Porphyrins in Mixtures Containing Zn-Proto
Let E refer to the Soret excitation of a fluorescent compound. Then let E420 R 592
570 f
and E420 R 620
ðÞ
represent the respective fluorescence integrals between
570 and 592 nm and between 592 and 620 nm for a mixture of Zn-Proto and
Mg-porphyrins, which are elicited by a 420 nm excitation of the hexane-extracted
acetone solution (Smith and Rebeiz 1977a ). The following simultaneous equations
can then be written for the fluorescent mixture:
d
λ
592 f
ðÞ
d
λ
E420 Z 620
592
f
ðλÞ
d
ðλÞ
Mg-Porphyrins E420 Z 620
592
Zn-Proto E420 Z 620
592
¼
f
ðλÞ
d
λ
þ
f
ðλÞ
d
λ
(3.27)
and
E 420 Z 592
570
f
ðλÞ
d
λ
Mg-porphyrins E420 Z 620
592
k 2 Zn-Proto E420
Z 620
¼ð
k 1 Þ
f
ðλÞ
d
λ
þ
ð
f
ðλÞ
d
λ
592
where:
E420 Z 592
570
E420 Z 620
592
k 1 ¼
Mg-Porphyrins
ð
f
ðλÞ
d
λ=
Mg-Porphyrins
ð
f
ðλÞ
d
ðλÞ
(3.28)
and,
E420 Z 592
570
E420 Z 620
592
k 2 ¼
Zn-Proto
ð
f
ðλÞ
d
λ=
Zn-Proto
ð
f
ðλÞ
d
ðλÞ
Where:
E420 Z 592
570
E420 Z 620
592
k 1 ¼
Mg-Porphyrins
ð
f
ðλÞ
d
λ=
Mg-Porphyrins
ð
f
ðλÞ
d
λÞ
(3.29)
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