Biology Reference
In-Depth Information
3.3.1 Calculation of the Fluorescence Integral Between
592 and 620 nm Which Is Contributed Solely by
Mg-Porphyrins in Mixtures Containing Zn-Proto
Let E refer to the Soret excitation of a fluorescent compound. Then let E420
R
592
570
f
and E420
R
620
ðÞ
represent the respective fluorescence integrals between
570 and 592 nm and between 592 and 620 nm for a mixture of Zn-Proto and
Mg-porphyrins, which are elicited by a 420 nm excitation of the hexane-extracted
acetone solution (Smith and Rebeiz
1977a
). The following simultaneous equations
can then be written for the fluorescent mixture:
d
λ
592
f
ðÞ
d
λ
E420
Z
620
592
f
ðλÞ
d
ðλÞ
Mg-Porphyrins
E420
Z
620
592
Zn-Proto E420
Z
620
592
¼
f
ðλÞ
d
λ
þ
f
ðλÞ
d
λ
(3.27)
and
E
420
Z
592
570
f
ðλÞ
d
λ
Mg-porphyrins
E420
Z
620
592
k
2
Zn-Proto
E420
Z
620
¼ð
k
1
Þ
f
ðλÞ
d
λ
þ
ð
f
ðλÞ
d
λ
592
where:
E420
Z
592
570
E420
Z
620
592
k
1
¼
Mg-Porphyrins
ð
f
ðλÞ
d
λ=
Mg-Porphyrins
ð
f
ðλÞ
d
ðλÞ
(3.28)
and,
E420
Z
592
570
E420
Z
620
592
k
2
¼
Zn-Proto
ð
f
ðλÞ
d
λ=
Zn-Proto
ð
f
ðλÞ
d
ðλÞ
Where:
E420
Z
592
570
E420
Z
620
592
k
1
¼
Mg-Porphyrins
ð
f
ðλÞ
d
λ=
Mg-Porphyrins
ð
f
ðλÞ
d
λÞ
(3.29)
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